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Gotham’s worst villains team up in the new trailer for Batman: Arkham Knight

Batman: Arkham Knight‘s new “Gotham is Mine” trailer sets the stage for Batman’s toughest fight yet in the upcoming conclusion to Rocksteady’s Arkham trilogy, due out in June 2015.

The Scarecrow, Batman’s psychedelic tormentor from previous Arkham games, has rallied Gotham’s worst villains together in a single plan to murder the Dark Knight and have their way with Gotham City. Batman standards Two-Face, The Penguin, The Riddler, and Harley Quinn all make appearances. Rocksteady is insistent that the Joker is still dead after the events of Arkham City, although we still don’t know the identity of the mysterious Arkham Knight, who is said to be an original creation of Rocksteady’s own design. Poison Ivy also narrates part of the trailer, filling in Batman on Scarecrow’s scheme (is she actually helping Batman, or is it all just part of the plan?).

Related: Watch Batman infiltrate Ace Chemicals in this Arkham Knight gameplay

The plot bears echoes of the famous Knightfall plot line from the comics in the early ’90s, wherein a group of Batman’s nemeses teamed up to engineer his death. In that story Bane is the ringleader, rather than the Scarecrow.

This marks Rocksteady’s return to the franchise after Warner Bros. Montreal took the reins for Batman: Arkham OriginsIts open Gotham is roughly five times the size of the game area in Arkham City, and features, for the first time, a drivable Batmobile that can be summoned anywhere to help you move around the huge city, which Scarecrow has emptied of civilians with terrorist threats.

Batman: Arkham Knight comes out for PlayStation 4, Windows, and Xbox One on June 2, 2015.