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First, lab-grown reptile skin — next, mutant ninja turtles?

Teenage Mutant Ninja Turtles
Image used with permission by copyright holder
Scientists have engineered reptile skin in the lab, the first time such a feat has been achieved for a non-mammal species. From the reconstructed tissue, which belongs to the endangered green turtle, the scientists grew a virus with hopes to better understand and fight the disease.

The ChHV5 virus is associated with tumors and damages the immune system in green turtles.

“It is the most important infectious disease affecting these turtles globally and we have good evidence that, when the severity of the tumors reach a certain stage of severity, animals become immunosuppressed, waste away, and die,” Theirry Work, a scientist at the U.S. Geological Survey and the lead author of the study, told Digital Trends.

Lab-grown reptile skin
Thierry Work, USGS
Thierry Work, USGS

In order to fight the disease, researchers typically decide to develop it in the lab, where they can study and manipulate it more easily. “Doing so allows you to better understand how the virus interacts with host cells and identify weak points in virus replication that could lead to intervention,” Work said.

Work and his team had previously tried and failed to grow the virus using a number of conventional methods. After noticing that the virus seemed to grow only in skin cells, he said, “we opted to have a go at trying to re-create the 3D structure of turtle skin in the lab to see if that would allow the virus to replicate and — lo — it worked.”

The virus’ sun-shaped replication center. Thierry Work, USGS. Public domain

To reconstruct the skin’s complex 3D structure, the researchers used both tumor cells and normal skin cells. While watching the virus develop, they saw it replicate in never-before-seen detail, including strange sun-shaped centers.

Although ultimately successful, the research was not without obstacles. For one thing, turtle skin is usually coated in layers of algae, bacteria, and barnacles that make cultured growth — which needs to be sterile — difficult. “We had to figure out ways to biopsy a turtle and coax skin cells to grow in a petri dish in a sterile system without bacterial contamination,” Work said. “We then had to figure out ways to grow the different cell types that comprise skin.”

With what they have learned in the lab, the scientists are now developing a blood test to detect the virus is present in turtles before they develop tumors.

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Dyllan Furness
Dyllan Furness is a freelance writer from Florida. He covers strange science and emerging tech for Digital Trends, focusing…
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