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CRISPR gene editing may have just become a whole lot more accurate

mcgill crispr 90 percent breakthrough wr 6 8 17
CRISPR gene editing is an enormously exciting area for molecular biologists, opening up new opportunities when it comes to editing DNA to fight back against a wide range of medical conditions. Heck, it’s so versatile that it can even be used to encode a GIF into the DNA of a virus, demonstrating how it is possible to capture complex information in living bacteria.

If CRISPR (clustered regularly interspaced short palindronic repeats) wasn’t already exciting enough, however, it could be about to become even more of a highly prized tool in the hands of researchers. At McGill University in Montreal, researchers in Uri David Akavia’s team have demonstrated CRISPR’s best success rate yet: managing to repair mutations in 90 percent of target cells.

That is particularly significant because, while CRISPR is very good at disabling certain genes (in one recent experiment it was able to disable the defective gene that triggers ALS in mice), it is much harder to use CRISPR to fix genes. This approach, which usually works in under 10 percent of target cells, involves replacing a faulty DNA with another working sequence. Until now, the highest success rate was 60 percent, meaning that the McGill University team has performed significantly better.

In order to increase the efficiency of the CRISPR technique, Akavia’s team physically linked the replacement DNA with the CRISPR protein which finds and removes the faulty sequence, as well as using a polymer called polyethyleneimine to directly target the nucleus of the cells — thereby reducing waste.

Going forward, the technique could conceivably be used for treating or eliminating entirely all inherited diseases. This is something previous CRISPR work has sought to do but could be revolutionized by this more successful approach. However, before too many champagne corks are popped, the team will need to demonstrate efficacy on a wide range of cells to show that their approach is versatile and repeatable under different conditions. We’ll keep our fingers firmly crossed!

“Our approach offers a cost-effective, simple and broadly applicable editing method, thereby expanding the CRISPR/Cas9 genome editing toolbox,” the researchers note in a paper describing the work. The research paper is available to read on biological sciences online repository, bioRxiv.

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